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Welso Cryogenic Grinding Solution for High-Integrity RNA

In molecular biology workflows, the quality of RNA extraction is highly dependent on the sample pre-treatment process. This is especially critical for mouse liver tissues, which are rich in RNases. Inadequate tissue disruption or poor temperature control can easily lead to RNA degradation, ultimately affecting downstream applications such as RT-qPCR and transcriptome sequencing.

As a provider of laboratory sample preparation solutions, Welso is committed to delivering efficient and reliable cryogenic grinding technologies that help preserve RNA integrity at the source, improving experimental success rates and data reliability.

Comparison: Cryogenic Grinding vs Conventional Methods

Objective

To achieve rapid and low-temperature homogenization of mouse liver tissue using Welso’s cryogenic grinding solution, effectively inhibiting RNase activity while improving RNA yield and integrity.

Cryogenic Grinding Workflow for Mouse Liver Tissue

Pre-cooling the Instrument
Activate the pre-cooling function of the Welso cryogenic grinder to bring the chamber to the target low temperature.

Sample Preparation
Place an appropriate amount of mouse liver tissue into a grinding tube and add stainless steel beads.

Rapid Loading
Once the system reaches the preset temperature, quickly load the sample adapter into the chamber.

Parameter Setup
Set grinding time, cycle numbers, and interval time (e.g., 5 seconds pause) based on sample characteristics to ensure efficient disruption.

Post-processing
Immediately remove the homogenized sample and proceed to the lysis step to preserve RNA integrity.

Experimental Results

Using the Welso cryogenic grinding solution, mouse liver tissue can be rapidly and uniformly homogenized within a short time. Compared to conventional methods, tissue disruption is more complete, significantly improving RNA release while minimizing degradation.

(Recommended: include before-and-after homogenization images for visualization)

Important Notes

Ensure Proper Pre-cooling: Only load samples after the system reaches the target temperature

Control Sample Size: Avoid overloading to maintain grinding efficiency

Minimize Room Temperature Exposure: Reduce RNA degradation risk

Immediate Lysis: Add lysis buffer immediately after grinding

Keep the Chamber Dry: Wipe off moisture after use to prevent icing and extend instrument lifespan

Key Advantages of Welso Cryogenic Grinding Solution

Welso integrates rapid freezing with high-efficiency mechanical grinding to deliver a robust solution for RNA sample preparation:

High Efficiency: Tissue disruption completed within seconds, increasing throughput

High Reproducibility: Excellent batch-to-batch consistency

High RNA Integrity: Low-temperature environment effectively inhibits RNase activity

Standardized Workflow: Fully controllable parameters for scalable laboratory operations

Critical Control Points

For optimal results, Welso recommends focusing on the following five key factors:

Rapid sampling · Thorough pre-cooling · Proper sample size · Short grinding time · Immediate lysis

By implementing Welso’s cryogenic grinding solution, laboratories can effectively prevent RNA degradation during sample preparation, consistently obtaining high-quality RNA for reliable downstream molecular biology applications.